Coding

Part:BBa_K363012:Design

Designed by: Justin Porter   Group: iGEM10_Johns_Hopkins   (2010-10-26)

Cnb1, the calcium sensitive regulatory subunit for calcineurin from S. cerevisiae


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This gene has been codon optimized for S. cerevisiae and has had restriction enzymes removed such that it is compatible with all five standards. It has also had an intron removed.

Source

This gene is from the eleventh chromosome of the yeast S. cerevisiae.

References

  • Cyert MS. Calcineurin signaling in Saccharomyces cerevisiae: how yeast go crazy in response to stress. Biochem Biophys Res Commun (2003)